doc. Mgr. Jan Paleček, Dr. rer. nat.

Curriculum vitae

Name, first names, scientific degrees

• doc. Mgr. Jan Paleček, Dr. rer. nat., 2nd of August 1966

Department/Faculty/University

• National Centre for Biomolecular Research – Faculty of Science
• Mendel Centre for Plant Genomics and Proteomics – Central European Institute of Technology

Function, current position

• Associate professor, group leader
• Guarantor of PhD programme Genomics and Proteomics
• Vice-Dean for Doctoral Studies

Education and academic qualifications

• 1985-1991: MSc. in biochemisty, Faculty of Science MU
• 1992-1998: Dr. rer. nat. in biochemisty, Biocentrum of Vienna University, Austria
• 2012-current: associate professor in molecular biology and genetics, Faculty of Science MU

Professional experience

• 1998-2002: junior researcher, Institute of Biofysics AS CR, Brno
• 2002-2006: senior researcher, GDSC, Sussex University, Great Britain
• 2006-2012: assistant professor, Faculty of Science MU
• 2012-current: senior researcher, CEITEC MU
• 2012-current: associate professor, Faculty of Science MU
• 2026-current: Vice-Dean for Doctoral Studies, Faculty of Science MU

Teaching activities

• Yeast biology (guarantor, from 2007)
• Structure and function of protein complexes (guarantor, from 2013)
• Proteomics (from 2013)
• Methods in proteomics (from 2013)

Research activities

• Analyses of protein-protein and DNA-protein interactions
• Studies of architecture and functions of protein complexes
• Studies of DNA repair mechanisms
• Studies of chromatin structure of yeast model

Professional stays abroad

• Short term stayes at Max Planck Institute in Gottingen and EMBL Heidelberg (Germany)
• PhD studies at Vienna University, Austria
• Postdoc at Sussex University, Great Britain

Other academic activities, research projects, grants

• 1999-2002: Role of domains and oligomeric state of p53 protein for its molecular interactions (301/99/D078)
• 2009-2011: Characterization of MAGE proteins: cofactors for E3 ubiquitin ligases? (IAA501630902)
• 2013-2016: Role of non-SMC proteins in stabilization and processing of stalled replication forks (P305-13-00774S)
• 2016-2018: Expressive visualization of protein complexes
• 2018–2020: Role of Nse1/Nse3/Nse4, E3-ubiquitin ligase, in genome stability (GA18-02067S)
• 2020–2022: Role of the SMC5/6 complex and its interaction partners in DNA damage repair (GA20-05095S)
• 2020–2023: The SMC5/6 complex in nucleome organization (LTC20033)
• 2023–2025: Deciphering the relationship between homologous recombination and gene silencing (GA23-05284S)
• 2026–2028: RAD60 - a new player in the maintenance of genome stability in plants (GA26-20390S)

Most significant professional accomplishments

• Editor of the General Physiology and Biophysics journal
• Member of the Scientific Board of the Institute of Biophysics AS CR (from 2022)
• Member of the Czech Society for Biochemistry and Molecular Biology (from 2021)
• Member of the British Biochemical Society (from 2022)

Major publications

• BAKOSOVA, Anetta; Lubos CIPAK; Nina MAYEROVA; Kamil KROL; Zsigmond BENKO; Alexandra PITELOVA; Peter KOLESÁR; Dominika PIATROVA; Maria SMONDRKOVA; Anna MARESOVA; Lucia MOLNAROVA; Ingrid CIPAKOVA; Veronika ALTMANNOVÁ; Jana BELLOVA; Peter BARATH; Martin PREVOROVSKY; Jan PALEČEK; Lumír KREJČÍ; Juraj GREGAN; Adrianna SKONECZNA a Silvia BAGELOVA POLAKOVA. Dbl2 interacts with helicases and an endonuclease to maintain the integrity of repetitive regions. Scientific Reports. Berlin: NATURE RESEARCH, 2025, roč. 15, č. 1, s. 1-18. ISSN 2045-2322. Dostupné z: https://doi.org/10.1038/s41598-025-08626-7. URL info
• FOJTA, Miroslav a Jan PALECEK. Professor Emil Paleček: seven decades with electrodes and biomolecules at the Institute of Biophysics of the CAS. EUROPEAN BIOPHYSICS JOURNAL. NEW YORK: SPRINGER, 2025, roč. 54, č. 8, s. 537-545. ISSN 0175-7571. Dostupné z: https://doi.org/10.1007/s00249-025-01771-x. URL info
• ANGELIS, Karel J.; Marcela HOLA; Radka VAGNEROVA; Jitka VACULÍKOVÁ a Jan PALECEK. The Phenotype of Physcomitrium patens SMC6 Mutant with Interrupted Hinge Interactions. Genes. BASEL: MDPI, 2025, roč. 16, č. 9, s. 1-15. ISSN 2073-4425. Dostupné z: https://doi.org/10.3390/genes16091091. URL info
• ŠTEFANOVIE, Barbora; Leon Paul JENNER; Lucie BOZDĚCHOVÁ; Petr FAJKUS; Eva SÝKOROVÁ; Jiří FAJKUS a Jan PALEČEK. Characterisation of the Arabidopsis thaliana telomerase TERT-TR complex. Plant Molecular Biology. Dordrecht (Netherlands): Springer, 2024, roč. 114, č. 3, s. 1-12. ISSN 0167-4412. Dostupné z: https://doi.org/10.1007/s11103-024-01461-w. URL info
• VACULÍKOVÁ, Jitka; Marcela HOLÁ; Barbora KRÁLOVÁ; Edit LELKES; Barbora ŠTEFANOVIE; Radka VÁGNEROVÁ; Karel J. ANGELIS a Jan PALEČEK. NSE5 subunit interacts with distant regions of the SMC arms in the Physcomitrium patens SMC5/6 complex. Plant Journal. Hoboken: Wiley, 2024, roč. 119, č. 3, s. 1481-1493. ISSN 0960-7412. Dostupné z: https://doi.org/10.1111/tpj.16869. URL info
• MAHRÍK, Lenka; Barbora ŠTEFANOVIE; Anna MARESOVA; Jarmila PRINCOVA; Peter KOLESÁR; Edit LELKES; Celline FAUX; Dominique HELMLINGER; Martin PREVOROVSKY a Jan PALEČEK. The SAGA histone acetyltransferase module targets SMC5/6 to specific genes. EPIGENETICS & CHROMATIN. LONDON: BMC, 2023, roč. 16, č. 1, s. 1-16. ISSN 1756-8935. Dostupné z: https://doi.org/10.1186/s13072-023-00480-z. URL info
• LELKES, Edit; Jitka JEMELKOVÁ; Marcela HOLÁ; Barbora ŠTEFANOVIE; Peter KOLESÁR; Radka VÁGNEROVÁ; Eva DVOŘÁK TOMAŠTÍKOVÁ; Aleš PEČINKA; Karel J. ANGELIS a Jan PALEČEK. Characterization of the conserved features of the NSE6 subunit of the Physcomitrium patens PpSMC5/6 complex. PLANT JOURNAL. ENGLAND: WILEY, 2023, roč. 115, č. 4, s. 1084-1099. ISSN 0960-7412. Dostupné z: https://doi.org/10.1111/tpj.16282. URL info
• KOLESÁR, Peter; Karel STEJSKAL; David POTĚŠIL; Johanne M MURRAY a Jan PALEČEK. Role of Nse1 Subunit of SMC5/6 Complex as a Ubiquitin Ligase. Cells. BASEL: MDPI, 2022, roč. 11, č. 1, s. 1-13. ISSN 2073-4409. Dostupné z: https://doi.org/10.3390/cells11010165. URL info
• VLADEJIC, Jovanka; Fen YANG; Eva Dvořák TOMAŠTÍKOVÁ; Jaroslav DOLEŽEL; Jan PALEČEK a Aleš PEČINKA. Analysis of BRCT5 domain-containing proteins reveals a new component of DNA damage repair in Arabidopsis. Frontiers in Plant Science. Lausanne: Frontiers Media S.A., 2022, roč. 13, December, s. 1-14. ISSN 1664-462X. Dostupné z: https://doi.org/10.3389/fpls.2022.1023358. URL info
• BYŠKA, Jan; Adam JURČÍK; Katarína FURMANOVÁ; Barbora KOZLÍKOVÁ a Jan PALEČEK. Visual Analysis of Protein–Protein Interaction Docking Models Using COZOID Tool. In Stefan Canzar, Francisca Rojas Ringeling. Protein-Protein Interaction Networks. 1. vyd. New York, NY: Humana Press Inc, 2020, s. 81-94. Methods in Molecular Biology, vol. 2074. ISBN 978-1-4939-9872-2. Dostupné z: https://doi.org/10.1007/978-1-4939-9873-9_7. URL info
• ADAMUS, Marek; Edit LELKES; David POTĚŠIL; Sri Ranjani GANJI; Peter KOLESÁR; Kateřina ZÁBRADY; Zbyněk ZDRÁHAL a Jan PALEČEK. Molecular insights into the architecture of the human SMC5/6 complex. Journal of Molecular Biology. London: Academic Press ltd-Elsevier Science ltd, 2020, roč. 432, č. 13, s. 3820-3837. ISSN 0022-2836. Dostupné z: https://doi.org/10.1016/j.jmb.2020.04.024. URL info
• VONDROVÁ, Lucie; Peter KOLESÁR; Marek ADAMUS; Matej NOCIAR; Antony William OLIVER a Jan PALEČEK. A role of the Nse4 kleisin and Nse1/Nse3 KITE subunits in the ATPase cycle of SMC5/6. Scientific reports. London: Nature Publishing Group, 2020, roč. 10, č. 1, s. 1-13. ISSN 2045-2322. Dostupné z: https://doi.org/10.1038/s41598-020-66647-w. URL info
• PALEČEK, Jan. SMC5/6: Multifunctional Player in Replication. GENES. BASEL: MDPI AG, 2019, roč. 10, č. 1, s. 1-14. ISSN 2073-4425. Dostupné z: https://doi.org/10.3390/genes10010007. URL info
• PALEČEK, Jan; Lucie VONDROVÁ; Kateřina ZÁBRADY a Jakub OTOČKA. Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes. In Badrinarayanan A. (eds). Methods in Molecular Biology. New York, NY: Springer Nature, 2019, s. 79-90. SMC Complexes, vol 2004. ISBN 978-1-4939-9519-6. Dostupné z: https://doi.org/10.1007/978-1-4939-9520-2_7. URL info
• FURMANOVÁ, Katarína; Jan BYŠKA; Eduard M. GRÖLLER; Ivan VIOLA; Jan PALEČEK a Barbora KOZLÍKOVÁ. COZOID: COntact ZOne IDentifier for visual analysis of protein-protein interactions. BMC Bioinformatics. 2018, roč. 19, APR, s. 1-17. ISSN 1471-2105. Dostupné z: https://doi.org/10.1186/s12859-018-2113-6. info
• ZÁBRADY, Kateřina; Marek ADAMUS; Lucie VONDROVÁ; Chunyan LIAO; Hana SKOUPILOVÁ; Markéta NOVÁKOVÁ; Lenka JURČIŠINOVÁ; Aaron ALT; Antony W. OLIVER; Alan R. LEHMANN a Jan PALEČEK. Chromatin association of the SMC5/6 complex is dependent on binding of its NSE3 subunit to DNA. Nucleic Acids Research. Oxford: Oxford University Press, 2016, roč. 44, č. 3, s. 1064-1079. ISSN 0305-1048. Dostupné z: https://doi.org/10.1093/nar/gkv1021. URL info
• VAN DER CRABBEN, Saskia; Marije HENNUS; Grant MCGREGOR; Deborah RITTER; Sandesh NAGAMANI; Owen WELLS; Magdalena HARAKALOVA; Ivan CHINN; Aaron ALT; Lucie VONDROVÁ; Ron HOCHSTENBACH; Joris VAN MONTFRANS; Suzanne TERHEGGEN-LAGRO; Stef VAN LIESHOUT; Markus VAN ROOSMALEN; Ivo RENKENS; Karen DURAN; Isaac NIJMAN; Wigard KLOOSTERMAN; Eric HENNEKAM; Jordan ORANGE; Peter VAN HASSELT; David WHEELER; Jan PALEČEK; Alan LEHMANN; Antony William OLIVER; Laurence PEARL; Sharon PLON; Johanne MURRAY a Gijs VAN HAAFTEN. Destabilized SMC5/6 complex leads to chromosome breakage syndrome with severe lung disease. Journal of Clinical Investigation. Ann Arbor: AMER SOC CLINICAL INVESTIGATION INC, 2016, roč. 126, č. 8, s. 2881-2892. ISSN 0021-9738. Dostupné z: https://doi.org/10.1172/JCI82890. URL info
• PALEČEK, Emil; Ján TKÁČ; Martin BARTOŠÍK; Tomáš BERTÓK; Veronika OSTATNÁ a Jan PALEČEK. Electrochemistry of Nonconjugated Proteins and Glycoproteins. Toward Sensors for Biomedicine and Glycomics. Chemical Reviews. Washington D.C.: American Chemical Society, 2015, roč. 115, č. 5, s. 2045-2108. ISSN 0009-2665. Dostupné z: https://doi.org/10.1021/cr500279h. URL info
• KOZÁKOVÁ, Lucie; Lucie VONDROVÁ; Karel STEJSKAL; Panagoula CHARALABOUS; Peter KOLESÁR; Alan R. LEHMANN; Stjepan ULDRIJAN; Christopher M. SANDERSON; Zbyněk ZDRÁHAL a Jan PALEČEK. The melanoma-associated antigen 1 (MAGEA1) protein stimulates the E3 ubiquitin-ligase activity of TRIM31 within a TRIM31-MAGEA1-NSE4 complex. Cell Cycle. Philadelphia: Taylor&Francis Inc, 2015, roč. 14, č. 6, s. 920-930. ISSN 1538-4101. Dostupné z: https://doi.org/10.1080/15384101.2014.1000112. URL info
• PALEČEK, Jan a Stephan GRUBER. Kite Proteins: a Superfamily of SMC/Kleisin Partners Conserved Across Bacteria, Archaea, and Eukaryotes. Structure. CAMBRIDGE: CELL PRESS, 2015, roč. 23, č. 12, s. 2183-2190. ISSN 0969-2126. Dostupné z: https://doi.org/10.1016/j.str.2015.10.004. URL info
• GUÉRINEAU, Marc; Zdeněk KŘÍŽ; Lucie KOZÁKOVÁ; Kateřina BEDNÁŘOVÁ; Pavel JANOŠ a Jan PALEČEK. Analysis of the Nse3/MAGE-binding Domain of the Nse4/EID Family Proteins. PLoS One. San Francisco: Public Library of Science, 2012, roč. 7, č. 4, s. "nestránkováno", 10 s. ISSN 1932-6203. Dostupné z: https://doi.org/10.1371/journal.pone.0035813. URL info
• HUDSON, J J R; Kateřina BEDNÁŘOVÁ; Lucie KOZÁKOVÁ; Chunyan LIAO; Marc GUÉRINEAU; Rita COLNAGHI; Susanne VIDOT; Jaromír MAREK; Sreenivas Reddy BATHULA; Alan LEHMANN a Jan PALEČEK. Interactions between the Nse3 and Nse4 Components of the SMC5-6 Complex Identify Evolutionarily Conserved Interactions between MAGE and EID Families. PloS ONE. San Francisco: Public Library of Science, 2011, roč. 6, č. 2, s. "nestránkováno", 14 s. ISSN 1932-6203. Dostupné z: https://doi.org/10.1371/journal.pone.0017270. URL info
• PROCHÁZKOVÁ SCHRUMPFOVÁ, Petra; Milan KUCHAŘ; Jan PALEČEK a Jiří FAJKUS. Mapping of interaction domains of putative telomere-binding proteins AtTRB1 and AtPOT1b from Arabidopsis thaliana. FEBS LETT. Netherlands: Elsevier Science BV, 2008, roč. 582, č. 10, s. 1400-1406. ISSN 0014-5793. info
• PALEČEK, Jan; Susanne VIDOT; Min FENG; Aidan DOHERTY a Alan LEHMANN. The Smc5-Smc6 DNA Repair Complex: Bridging of the SMC5-SMC6 heads by the kleisin, Nse4 ... Journal of Biological Chemistry. 2006, roč. 281, č. 48, s. 36952-9. URL info
• SERGEANT, John; Elaine TAYLOR; Jan PALECEK; Maria FOUSTERI; Sara SWEENEY; Hideo SHINAGAWA; Felicity WATTS a Alan LEHMANN. Composition and architecture of the Schizosaccharomyces pombe Rad18 (Smc5-6) complex. Molecular and Cellular Biology. 2005, roč. 25, s. 172-84. ISSN 0270-7306. info
• ANDREWS, Emily; Jan PALECEK; John SERGEANT; Elaine TAYLOR; Felicity WATTS a Alan LEHMANN. Nse2, a component of the Smc5-6 complex, is a SUMO ligase required for the response to DNA damage. Molecular and Cellular Biology. 2005, roč. 25, s. 185-96. ISSN 0270-7306. info
• FOJTA, Miroslav; Hana PIVOŇKOVÁ; Marie BRÁZDOVÁ; K NEMCOVA; Jan PALEČEK a Bořivoj VOJTĚŠEK. Investigations of the supercoil-selective DNA binding of wild type p53 suggest a novel mechanism for controlling p53 function. European Journal of Biochemistry. 2004, roč. 271, č. 19, s. 3865-3876. ISSN 0014-2956. info
• BRÁZDOVÁ, Marie; Jan PALEČEK; D.I. CHERNY; Sabina BILLOVÁ; Miroslav FOJTA; Petr PEČINKA; Bořivoj VOJTĚŠEK; T.M. JOVIN a Emil PALEČEK. Role of tumor suppressor p53 domains in selective binding to supercoiled DNA. Nucleic Acids Research. 2002, roč. 30, s. 4966-4974. info
• PALEČEK, Jan; J HASEK a H RUIS. Rpg1p/Tif32p, a subunit of translation initiation factor 3, interacts with actin-associated protein Sla2p. Biochemical and biophysical research communications. 2001, roč. 282, č. 5, s. 1244-1250. ISSN 0006-291X. info
• PALEČEK, Emil; Marie BRÁZDOVÁ; Václav BRÁZDA; Jan PALEČEK; Sabina BILLOVÁ; V SUBRAMANIAM a T M JOVIN. Binding of p53 and its core domain to supercoiled DNA. European Journal of Biochemistry. 2001, roč. 268, č. 3, s. 573-581. ISSN 0014-2956. info
• BRÁZDA, Václav; Jan PALEČEK; Šárka POSPÍŠILOVÁ; Bořivoj VOJTĚŠEK a Emil PALEČEK. Specific modulation of p53 binding to consensus sequence within supercoiled DNA by monoclonal antibodies. Biochemical and biophysical research communications. 2000, roč. 267, č. 3, s. 934-939. ISSN 0006-291X. info
• WESP, A; L HICKE; Jan PALEČEK; R LOMBARDI; Tina HAUSTEIN; A L MUNN a H RIEZMAN. End4p/Sla2p interacts with actin-associated proteins for endocytosis in Saccharomyces cerevisiae. Molecular Biology of the Cell. 1997, roč. 8, č. 11, s. 2291-2306. ISSN 1059-1524. info

2026/02/04